Seminalplasmin, the major basic protein of bull seminal plasma, is a secretory protein of the seminal vesicles

Seminalplasmin, the major basic protein of bull seminal plasma, is a secretory protein of the seminal vesicles.

From the experimental results of three independent methods: (1) indirect immunofluorescence employing monospecific anti-seminalplasmin-IgGs, (2) cell-free translation of poly(A)+ RNA from seminal vesicle and testicular tissue, as well as (3) Northern analysis of poly(A)+ RNA of the latter tissues with a synthetic seminalplasmin-specific antisense DNA probe, it is concluded that the biosynthesis...

Interaction of PDC-109, the major secretory protein from bull seminal vesicles, with bovine sperm membrane Ca2+-ATPase.

PDC-109 is the prevalent secretory protein from bovine seminal vesicles that binds to the midpiece of sperm once they pass the ampulla of the vas deferens during emission. Thereby, the protein changes biophysical membrane properties, eventually resulting in increased sperm motility. To elucidate the underlying biochemical mechanism, we have studied the ion-pumping activity (Ca(2+)-ATPase) in me...

The Effect of Cattle Seminal Plasma Crude Protein on the Cryopreservation of Goat Semen

This study aimed to improve the quality of cryopreserved goat semen for artificial insemination by the addition of cattle seminal plasma crude protein. Semen collected from cattle and centrifuged to obtain seminal plasma. Then it was purified to obtain the crude protein. The diluent used in this study was milk-yolk. There were three groups, control group without crude protein (P0), the addition...

The major protein of bull seminal plasma: biosynthesis and biological function.

We isolated the major protein of apparent Mr of 15,000-16,000 from seminal plasma as well as from seminal vesicle secretion of bull and proved by amino acid analysis and tryptic peptide mapping that the two proteins were identical. An antiserum against this major protein was employed to quantitate and identify the major protein in seminal plasma as well as seminal vesicle secretion. The antiser...

Constituents of Bull Seminal Plasma